Primer

Part:BBa_K3385019:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-13)


spaA_repair_oligo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 82
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The repair oligo fits the regions just outside the cutting areas, for the repair of the DNA after cutting.

Source

The spaA sequence was found in the Aspergillus genome database, aspgd.org [1].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.